Figure 5.
Analysis of CARNMT1 knock-out cells. (A) Table showing the secondary screen CRISPR score of CARNMT1 iKO in HeLa and K562 cells. (B) Representative confocal images of mitotic and interphase HeLa cells showing the localization of GFP-tagged CARNMT1. Scale bar: 2 µm. (C) Representative Z-projected deconvolved images of control or CARNMT1 iKO K562 cells with nocodazole, paclitaxel, or STLC. Microtubules (DM1α), DNA (DAPI). Scale bar: 9 µm. (D) Percentage of mitotic cells without mitotic defects in control or CARNMT1 iKO, treated with nocodazole (noc) or paclitaxel (pac). n > 300 cells per condition, across three experiments. (E) Percentage of cells displaying monopolar spindle in control or CARNMT1 iKO in STLC. n > 300 cells per condition, across three experiments. (F) Quantification of total spindle tubulin immunofluorescence signal in control or CARNMT1 iKO in K562 s. n = 58, 64 across three experimental replicates. For representative images, see Fig. S3 K. (G) Quantification of total spindle tubulin immunofluorescence in the CARNMT1 iKO K562 cells treated with nocodazole. n = 62, 63 across three experimental replicates. (H) Quantification of total spindle tubulin immunofluorescence in the CARNMT1 iKO K562 cells treated with paclitaxel. n = 62, 61 across three experimental replicates. Statistical tests performed: Welch’s t test (ns = not significant, ****p = <0.0001). Blue lines indicate the median.

Analysis of CARNMT1 knock-out cells. (A) Table showing the secondary screen CRISPR score of CARNMT1 iKO in HeLa and K562 cells. (B) Representative confocal images of mitotic and interphase HeLa cells showing the localization of GFP-tagged CARNMT1. Scale bar: 2 µm. (C) Representative Z-projected deconvolved images of control or CARNMT1 iKO K562 cells with nocodazole, paclitaxel, or STLC. Microtubules (DM1α), DNA (DAPI). Scale bar: 9 µm. (D) Percentage of mitotic cells without mitotic defects in control or CARNMT1 iKO, treated with nocodazole (noc) or paclitaxel (pac). n > 300 cells per condition, across three experiments. (E) Percentage of cells displaying monopolar spindle in control or CARNMT1 iKO in STLC. n > 300 cells per condition, across three experiments. (F) Quantification of total spindle tubulin immunofluorescence signal in control or CARNMT1 iKO in K562 s. n = 58, 64 across three experimental replicates. For representative images, see Fig. S3 K. (G) Quantification of total spindle tubulin immunofluorescence in the CARNMT1 iKO K562 cells treated with nocodazole. n = 62, 63 across three experimental replicates. (H) Quantification of total spindle tubulin immunofluorescence in the CARNMT1 iKO K562 cells treated with paclitaxel. n = 62, 61 across three experimental replicates. Statistical tests performed: Welch’s t test (ns = not significant, ****p = <0.0001). Blue lines indicate the median.

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