Visualization of additional screen output. (A) Scatter plot illustrating the differential CRISPR scores across all gene targets in the primary screen. The differential was calculated between nocodazole and untreated and paclitaxel and untreated K562 cell pools. (B) Autosomal start and end positions (from GRCH38.p12) of genes (black lines) with CRISPR score >1.5, P < 0.05 in 250 nM nocodazole (suppressors) were obtained from BioMart and plotted in R. (C) Scatter plot showing the CRISPR scores in untreated versus nocodazole treated HeLa cell pools from the secondary screen. (D) Scatter plot showing the CRISPR scores in untreated versus paclitaxel-treated HeLa cell pools from the secondary screen. (E) Scatter plot showing the CRISPR scores in untreated versus STLC-treated HeLa cell pools from the secondary screen. (F and G) Scatter plots showing the differential CRISPR scores in treated versus untreated HeLa and K562 cells. The differential was calculated between nocodazole and untreated (F) and paclitaxel and untreated cell pools (G). (H) Scatter plot showing the CRISPR scores in untreated versus nocodazole-treated K562 cell pools. (I) Scatter plot showing the CRISPR scores in untreated versus paclitaxel treated K562 cell pools. (J) Scatter plot showing the CRISPR scores in untreated versus STLC treated K562 cell pools.