Figure 3. The Nap1 2 β-hairpin and... | Rockefeller University Press

Figure 3.

The Nap12β-hairpin and Kap114 h19loop are binding hotspots. (A) Pull-down assay with equimolar MBP-Kap114 (1 µM), Nap12 (WT, β-hairpin mutant βERQ (E288A/R290A/Q292A) or histone-binding site mutant α5/6mut (E194A/D201A/D205A), as indicated by schematic on the left) ± H2A-H2B ± RanGTP. Bounds proteins were visualized by Coomassie-stained SDS-PAGE. Controls in Fig. S4 C. (B) Kap114 h19loop mutants Δh19L (h19loop deleted), YDAA (Y939A/D942A), and DDAA (D928A/D929A) in pull-down assay as in A. (C) Summary of pull-down assays with immobilized MBP-Kap114 (iK). Source data are available for this figure: SourceData F3.

The Nap1 ₂ β-hairpin and Kap114 h19loop are binding hotspots. (A) Pull-down assay with equimolar MBP-Kap114 (1 µM), Nap1₂ (WT, β-hairpin mutant β_ERQ (E288A/R290A/Q292A) or histone-binding site mutant α5/6_mut (E194A/D201A/D205A), as indicated by schematic on the left) ± H2A-H2B ± Ran^GTP. Bounds proteins were visualized by Coomassie-stained SDS-PAGE. Controls in Fig. S4 C. (B) Kap114 h19loop mutants Δh19L (h19loop deleted), YD_AA (Y939A/D942A), and DD_AA (D928A/D929A) in pull-down assay as in A. (C) Summary of pull-down assays with immobilized MBP-Kap114 (iK). Source data are available for this figure: SourceData F3.

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