Nsun2 regulates the expression of cytokines in ILC3s. (A) LPLs from the small intestinal and colonic lamina propria of Rorc^creNsun2^fl/fl or Nsun2^fl/fl littermate mice were stimulated with IL-23 in vitro. The production of IL-22 and GM-CSF by ILC3s (gating in eflour780⁻CD45^lowCD90⁺) was analyzed by flow cytometry. (B) LPLs from the small intestinal and colonic lamina propria of Rorc^creNsun2^fl/fl or Nsun2^fl/fl littermate mice were stimulated with PMA/Ionomycin in vitro. The production of IL-17A and IFNγ by ILC3s (gating in eflour780⁻CD45^lowCD90⁺) was analyzed by flow cytometry. (C) ILC3s were sorted from the small intestinal and colonic LPLs of Rorc^creNsun2^fl/fl or Nsun2^fl/fl littermate mice. The mRNA expression of Il1r1, Il1r2, Il1rap, Dusp1, Dusp4 and Dusp8 was measured by qPCR. Data are representative of one of two independent experiments (A–C). Data are mean ± SEM and two-tailed Student’s unpaired t test; ns, no significance; *P < 0.05, ***P < 0.001, ****P < 0.0001.