Nsun2 in ILC3s is required for the cytokine production and host protection against colitis. (A) ILC3s (gated in CD45⁺CD90⁺CD3⁻RORγt⁺) were isolated from colonic LPLs of Nsun2^fl/fl (n = 5) and Rorc^creNsun2^fl/fl (n = 4) mice and analyzed by flow cytometry. The percentages and absolute numbers of ILC3s are shown. (B) LPLs from colonic lamina propria of Rorc^creNsun2^fl/fl (n = 4) or Nsun2^fl/fl (n = 5) littermate mice were stimulated ex vivo with IL-1β or PMA/ionomycin (P/I). Representative flow cytometry and analysis of IL-22 and GM-CSF production by ILC3s (gating in eflour780⁻CD45^lowCD90⁺ RORγt⁺). (C) 8-wk-old Rorc^creNsun2^fl/fl (n = 7) and their littermates Nsun2^fl/fl (n = 8) were given 3% DSS in drinking water for 6 days. Body weight change curves were shown. Data are pooled from two independent experiments. (D) The IL-22 and GM-CSF production by ILC3s (gating in CD45⁺CD90⁺TCRβ⁻RORγt⁺) from Rorc^creNsun2^fl/fl (n = 5) or Nsun2^fl/fl (n = 4) mice at day 3 after DSS exposure. (E) 8-wk-old Cd4^creNsun2^fl/fl (n = 3) and their littermates Nsun2^fl/fl (n = 4) were given 3% DSS in drinking water for 6 days. Body weight change curves were shown. Data are representative of one of two independent experiments (A–E). Each dot (A, B, and D) represents one individual mouse. Data are mean ± SEM and statistical significance was tested by two-tailed Student’s unpaired t test (A–E), *P < 0.05, **P < 0.01, ****P < 0.0001; ns, no significant difference.