p38α regulates the cytokine expression in ILC3s through eIF6. (A) MK2 inhibition didn’t affect ILC3 cytokine production. LPLs were pretreated with SB203580 and CMPD1 and then stimulated with IL-1β in vitro. IL-22 and GM-CSF production by ILC3s (gated in eflour780⁻CD45^lowCD90^high) were examined by flow cytometry. Data are representative of one of two independent experiments. Data are mean ± SEM and statistical significance was tested by two-tailed Student’s unpaired t test; ****P < 0.0001; ns, no significant difference. (B) IL-1β treatment did not influence the IL-22 and GM-CSF mRNA splicing in ILC3s. The diagram for primer design to test maturation of Il22 and Csf2 mRNA. The mature or intron-contained premature mRNA was determined by RT-PCR and agarose gel electrophoresis. One of two independent experiments is shown. (C) The size exclusion chromatogram of purified eIF6. eIF6 displayed a significant advance in elution from the column compared with the predicted elution volume (indicated by arrow). One of two independent experiments is shown. (D) IL-1β promotes m⁵C modification in MNK-3 cells. RNA dot blot analysis of mRNAs with m⁵C modification in MNK-3 cells with or without IL-1β treatment. Total RNA was extracted from MNK-3 cells, crosslinked to the NC membrane, and detected by anti-m⁵C antibody. The data are representative of at least two independent experiments. Source data are available for this figure: SourceData FS3.