p38α in ILC3s is crucial for maintaining intestinal homeostasis and preventing intestinal inflammation. (A–C) 8-wk-old mice were exposed to 3% DSS for 6 days. (A) Body weight change curves of DSS-treated (RorccreMapk14fl/fl, n = 13; Mapk14fl/fl, n = 13) or control mice (RorccreMapk14fl/fl, n = 9; Mapk14fl/fl, n = 10) were shown. (B) The colon length of DSS-treated (RorccreMapk14fl/fl, n = 7; Mapk14fl/fl, n = 5) or control mice (RorccreMapk14fl/fl, n = 4; Mapk14fl/fl, n = 3) were measured. (C) The colons of RorccreMapk14fl/fl (n = 6) and Mapk14fl/fl mice (n = 6) were collected for H&E staining at day 10 after DSS treatment. Gross morphological changes of colons and colitis score were shown. Scale bars, 250 μm. (D) Representative flow cytometry and pooled analysis of IL-22 and GM-CSF production by ILC3s (gating in CD45+CD90+TCRβ−RORγt+) from colonic LPLs of RorccreMapk14fl/fl (n = 4) or Mapk14fl/fl (n = 4) littermate mice after 3 days of DSS exposure. (E and F)Cd4creMapk14fl/fl (n = 7) and Mapk14fl/fl (n = 7) mice were given 3% DSS in drinking water for 6 days. Body weight change curves (E) and colon length (F) were shown. (G and H) 8-wk-old Mapk14fl/fl or RorccreMapk14fl/fl mice were administrated with PBS or IL-22 and treated with 3% DSS for 6 days (PBS-RorccreMapk14fl/fl, n = 3; PBS-RorccreMapk14fl/fl, n = 4, Mapk14fl/fl, n = 6). Body weight changes (G) and the colon lengths (H) were shown. (I) Representative colonic histology and colitis score of naïve RorccreMapk14fl/fl (n = 4) and littermate mice (n = 4) around 20-wk old. Scale bars, 250 μm. Data are representative of one of two independent experiments (D, G, and H). Two experiments were pooled together (A, C, E, and I). Each dot (B–I) represents one individual mouse. Data are mean ± SEM, and two-tailed Student’s unpaired t test (A–I) were used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001; ns, no significant difference.
Figure 2.

p38α in ILC3s is crucial for maintaining intestinal homeostasis and preventing intestinal inflammation. (A–C) 8-wk-old mice were exposed to 3% DSS for 6 days. (A) Body weight change curves of DSS-treated (RorccreMapk14fl/fl, n = 13; Mapk14fl/fl, n = 13) or control mice (RorccreMapk14fl/fl, n = 9; Mapk14fl/fl, n = 10) were shown. (B) The colon length of DSS-treated (RorccreMapk14fl/fl, n = 7; Mapk14fl/fl, n = 5) or control mice (RorccreMapk14fl/fl, n = 4; Mapk14fl/fl, n = 3) were measured. (C) The colons of RorccreMapk14fl/fl (n = 6) and Mapk14fl/fl mice (n = 6) were collected for H&E staining at day 10 after DSS treatment. Gross morphological changes of colons and colitis score were shown. Scale bars, 250 μm. (D) Representative flow cytometry and pooled analysis of IL-22 and GM-CSF production by ILC3s (gating in CD45+CD90+TCRβ−RORγt+) from colonic LPLs of RorccreMapk14fl/fl (n = 4) or Mapk14fl/fl (n = 4) littermate mice after 3 days of DSS exposure. (E and F)Cd4creMapk14fl/fl (n = 7) and Mapk14fl/fl (n = 7) mice were given 3% DSS in drinking water for 6 days. Body weight change curves (E) and colon length (F) were shown. (G and H) 8-wk-old Mapk14fl/fl or RorccreMapk14fl/fl mice were administrated with PBS or IL-22 and treated with 3% DSS for 6 days (PBS-RorccreMapk14fl/fl, n = 3; PBS-RorccreMapk14fl/fl, n = 4, Mapk14fl/fl, n = 6). Body weight changes (G) and the colon lengths (H) were shown. (I) Representative colonic histology and colitis score of naïve RorccreMapk14fl/fl (n = 4) and littermate mice (n = 4) around 20-wk old. Scale bars, 250 μm. Data are representative of one of two independent experiments (D, G, and H). Two experiments were pooled together (A, C, E, and I). Each dot (B–I) represents one individual mouse. Data are mean ± SEM, and two-tailed Student’s unpaired t test (A–I) were used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001; ns, no significant difference.

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