Flow cytometry data for the functional validation of BTNL8 variants. (A and B) Example flow cytometry plots for (A) the surface expression of BTNL3 and BTNL8 48 h posttransfection of 293T cells transfected with the indicated plasmid quantities and (B) CD3 and CD69 expression on JRT3 cells expressing a Vγ4Vδ1 TCR (clone hu17) following a 5 h co-culture with transfected 293T cells. (C) FLAG-BTNL3–specific gMFI, HA-BTNL8 specific gMFI, TCR downregulation (as % of control) and CD69 upregulation (fold relative to control) for all BTNL8 variants tested.