Structural modeling of the BTNL8/BTNL3 complex. (A) Schematic representation of the BTNL8/BTNL3 heterodimer. The intracellular B30.2 domain facilitates the stabilization of the complex. (B) AlphaFold-generated 3D structure prediction of the complex, visualized with Mol*. The B30.2 domain is highlighted in the green box and the location of all the variants detected in the cohort is highlighted in yellow. (C) RIN analysis of the B30.2 domains. Nodes in the graph correspond to residues and edges correspond to residue-residue interactions. The network is derived from the 3D structure and takes into account geometric parameters and known physico-chemical properties. The resulting graph is a 2D projection of the underlying structure, with yellow nodes indicating the positions of MIS-C variants and edge colors representing different types of interaction. (D) Structural modeling of B30.2 variants. AlphaFold-predicted structures for each variant (i–vi) are superimposed on the reference structure. Reference residues and corresponding 3D planes are depicted in orange, while variants are in magenta.