Illustrates structural rearrangements of the SPC upon removal of Spc2. (A) Crossing angle computed from the vector defined in the Spc3 TM helix 1, between residues 4 and 23, and the vector defined in the Spc1 TM helix 1, between residues 20 and 43. Single-point cross-angles calculated from the AF2 structures are shown in red vertical lines. (B) Representative snapshots illustrating the SPC WT (left) and spc2Δ (right) structures. The phosphate headgroups are colored in salmon (WT) or yellow (spc2Δ), Spc2 in green, Sec11 in purple, Spc3 in red, Spc1 in orange, and the lipid tails are represented as a transparent grey surface.