Provides model validation for the MD simulations. (A and B) Comparison of the α-carbon root mean squared fluctuations (RMSFs) between all-atom (AA) and coarse-grained (CG) MD simulations in a POPC bilayer, per subunit. (A) WT SPC and (B) SPC lacking Spc2 (spc2Δ). Sec11 is shown in purple, Spc3 in red, Spc1 in orange, Spc2 in green, the atomistic reference RMSF is colored in blue and error bars are shown in grey. (C) iPTM scores for each model predicted by AlphaFold2-Multimer (V3) for the tetramer and trimer (spc2Δ) SPC. (D) RMSD time-series extracted from all-atom MD simulations of the wild-type and trimeric variants of yeast SPC embedded in a POPC bilayer. The different colors correspond to individual 1 μs long simulation runs and the black line is an average RMSD computed from the five repeats.