Shows the predicted signal sequence cleavage sites of the proteins used in the study. (A) The N-terminal 60 residues of Pho8 and Pho8(P54A) are shown. Signal-anchored sequences are underlined. (B–D) SignalP (Almagro Armenteros et al., 2019) predictions for Pho8 and Pho8(P54A) (B), CPY, N#CPYt(h) CS1/2, CS1 and CS2 (C). Rrt6 (CS1-like), Rrt6_T48Q,L52A,D55P (CS2-like), Rrt6_L52A (CS1/2-like) (D).