Figure S1.
Presents sequences and data related to Spc2, its homologs and mutants. (A) Sequence alignment of Spc2 homologs (human, canine and S. cerevisiae). Predicted TMs are in yellow. Underlined sequences were replaced with the 4L/15A hydrophobic segment in Spc2-TM2* (shown in magenta). Orange colored sequences were truncated in Spc2-ΔCD(58) and orange colored sequences in italic were truncated in Spc2-ΔCD(23). (B and C) The volcano plots comparing the proteomes from spc2Δ cells carrying SPC2 and spc2-ΔCD(58) (B) or SPC2 and spc2-TM2* (C) as quantified from mass spectrometry (Data S2). The relative abundances of Sec11, Spc3, Spc2, and Sbh2 are indicated in green circles, and Pdi1 and Kar2 in red square. (D) Cleavage of SPSuc2-Lep (Hessa et al., 2009) and ppαF in the spc2Δ, SPC3HA strain carrying an empty vector (EV), SPC2, spc2-ΔCD(58), and spc2-TM2* was analyzed by pulse labeling. Source data are available for this figure: SourceData FS1.

Presents sequences and data related to Spc2, its homologs and mutants. (A) Sequence alignment of Spc2 homologs (human, canine and S. cerevisiae). Predicted TMs are in yellow. Underlined sequences were replaced with the 4L/15A hydrophobic segment in Spc2-TM2* (shown in magenta). Orange colored sequences were truncated in Spc2-ΔCD(58) and orange colored sequences in italic were truncated in Spc2-ΔCD(23). (B and C) The volcano plots comparing the proteomes from spc2Δ cells carrying SPC2 and spc2-ΔCD(58) (B) or SPC2 and spc2-TM2* (C) as quantified from mass spectrometry (Data S2). The relative abundances of Sec11, Spc3, Spc2, and Sbh2 are indicated in green circles, and Pdi1 and Kar2 in red square. (D) Cleavage of SPSuc2-Lep (Hessa et al., 2009) and ppαF in the spc2Δ, SPC3HA strain carrying an empty vector (EV), SPC2, spc2-ΔCD(58), and spc2-TM2* was analyzed by pulse labeling. Source data are available for this figure: SourceData FS1.

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