Expression of ITPR members in HD and patient cells. (A) RT-qPCR for ITPR1, ITPR2, and ITPR3 transcripts in peripheral blood T cells isolated from HD and patients (P2, P4, and P5). Results shown are relative to beta-actin (ACTB) expression, are representative of two independent experiments, and are not statistically significant via multiple unpaired t tests with Holm-Sidak correction for multiple comparisons. (B) Sanger sequencing of cDNA showing expression of WT and variant ITPR3 transcripts for P1 (fibroblasts), P2 (T cells), P3 (LCLs), P4 (T cells), and P5 (T cells); REF = reference sequence, ALT = alternative sequence; representative of two independent experiments. (C) Protein expression of ITPR3 in LCLs from HDs, P3 and P4. (I) Western blot for ITPR3 and GAPDH using LCL lysates; representative of three independent experiments. (II) Densitometric quantification of ITPR3 protein abundance in LCLs relative to GAPDH. (D) Western blot for ITPR3 and GAPDH in ITPR3 KO HEK293T cells (ITPR3 KO) and in transfected ITPR3 KO cells expressing WT ITPR3 protein or the A196T, I2506N, and R2524C protein variants; representative of two independent transfection experiments. Source data are available for this figure: SourceData F2.