Figure 6.
Comparative analysis of ER-phagy and ER network in developing and adult tissues. (A) Representative images of pancreas sections at postnatal day 9 (P9), P19, and in adulthood from ER-TRG (+/−) reporter mice. Scale bar: 20 μm. (B) Quantitative analysis of ER-phagy in pancreas from ER-TRG (+/−) reporter mice at P9, P19, and Adult. Confocal images in 6A were quantified. Values were stated as mean ± SEM (n = 4–5 per group). Statistical differences were evaluated using one-way ANOVA. ***P < 0.001. (C) Representative images of kidney sections at P9, P19, and in adulthood from ER-TRG (+/−) reporter mice (n = 4 per group). Scale bar: 20 μm. (D–G) Immunoblot and quantitative analysis showing ER-phagy activities in pancreas and kidney isolated from ER-TRG (+/−) reporter at P9, P19, Adult. The band intensities of RFP and RFP-GFP were quantified and the ratio of RFP:RFP-GFP (normalized to WT) is shown (F and G). Values were stated as mean ± SEM (n = 4–5 per group). Statistical differences were evaluated using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. (H) Schematic representation of the intestinal villus and crypt structure, including cell identities. The mature epithelium comprises goblet cells and enterocytes, while the crypt contains stem cells and Paneth cells in the transit amplifying (TA) zone. (I and J) Representative images of small intestine sections and quantitative analysis of ER-phagy activities in sub-regions at P9 and in adulthood from ER-TRG (+/−) reporter mice. A magnified view highlights RFP-only signals in the cells of the intestinal villus. Values were stated as mean ± SEM (n = 3–5 per group). Statistical differences were evaluated using two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar: 20 μm. Source data are available for this figure: SourceData F6.

Comparative analysis of ER-phagy and ER network in developing and adult tissues. (A) Representative images of pancreas sections at postnatal day 9 (P9), P19, and in adulthood from ER-TRG (+/−) reporter mice. Scale bar: 20 μm. (B) Quantitative analysis of ER-phagy in pancreas from ER-TRG (+/−) reporter mice at P9, P19, and Adult. Confocal images in 6A were quantified. Values were stated as mean ± SEM (n = 4–5 per group). Statistical differences were evaluated using one-way ANOVA. ***P < 0.001. (C) Representative images of kidney sections at P9, P19, and in adulthood from ER-TRG (+/−) reporter mice (n = 4 per group). Scale bar: 20 μm. (D–G) Immunoblot and quantitative analysis showing ER-phagy activities in pancreas and kidney isolated from ER-TRG (+/−) reporter at P9, P19, Adult. The band intensities of RFP and RFP-GFP were quantified and the ratio of RFP:RFP-GFP (normalized to WT) is shown (F and G). Values were stated as mean ± SEM (n = 4–5 per group). Statistical differences were evaluated using one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. (H) Schematic representation of the intestinal villus and crypt structure, including cell identities. The mature epithelium comprises goblet cells and enterocytes, while the crypt contains stem cells and Paneth cells in the transit amplifying (TA) zone. (I and J) Representative images of small intestine sections and quantitative analysis of ER-phagy activities in sub-regions at P9 and in adulthood from ER-TRG (+/−) reporter mice. A magnified view highlights RFP-only signals in the cells of the intestinal villus. Values were stated as mean ± SEM (n = 3–5 per group). Statistical differences were evaluated using two-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar: 20 μm. Source data are available for this figure: SourceData F6.

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