Autophagy-deficient Langerhans cells undergo ferroptosis. (A) Differentially expressed transcripts included in the KEGG ferroptosis pathway (mmu04216) in Atg5^WT versus Atg5^ΔCd207 LCs. No threshold was applied to fold changes. (B) MFI of CD71 at the surface of LCs. (C) MFI of FerroOrange staining, reflecting the concentration of ferrous iron (Fe²⁺) in LCs. Data are pooled from five independent experiments, with each point representing one individual 3-wk-old mouse. (D) Quantification of lipid peroxidation for epidermal LCs of control (Atg5^WT and Atg5^WT/Δ) and Atg5^ΔCd207 mice, as measured by MFI of Bodipy-C11. Data are pooled from 10 independent experiments, with each point representing one individual 3-wk-old mouse. (E) Lipid peroxidation of Atg5^WT and Atg5^ΔCd207 LCs after overnight incubation with 50 µM ferrostatin-1. Data are pooled from four independent experiments, with each point representing one individual 3-wk-old mouse. Statistical analyses: (B–D) Kruskal–Wallis one-way ANOVA followed by Dunn’s multiple comparison test (***, P < 0.001; **, P < 0.01; *, P < 0.05; ns, P > 0.05). (E) Two-way ANOVA followed by Sidak’s multiple comparison tests. (*, P < 0.05; ns, P > 0.05).