The PI3K/Akt pathway is promoted in ATG5-deficient Langerhans cells. (A–F) MFI of (A and D) phosphorylated Akt, (B and E) phosphorylated S6, and (C and F) phosphorylated 4E-BP1 in epidermal mature LCs (A–C) or KCs (D–F) obtained from control (Atg5^WT and Atg5^WT/Δ) and Atg5^ΔCd207 mice, quantified by flow cytometry. Data are pooled from three independent experiments, with each point representing one individual 3-wk-old mouse. Statistical analysis: Kruskal–Wallis one-way ANOVA followed by Dunn’s multiple comparison test (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, P > 0.05).