Lack of autophagy alters the transcriptome of Langerhans cells. (A) Visualization of the exon 3 region of Atg5 gene from RNA-seq of sorted LCs of indicated mouse genotype using integrative Genomic Viewer tool. (B) Principal component analysis of RNA-seq transcriptome analysis from sorted LCs of Atg5^WT and Atg5^ΔCd207 mice. (C) Heatmap showing the differentially expressed genes (FDR < 0.1, Absolute Log₂ Fold Change value >1, P value <0.05) between LCs of indicated mouse genotypes. (D) Volcano plot showing the differential expression of genes between LCs of indicated mouse genotypes. Gene names refer to the top 20 up and downregulated genes, based on the following combinations of P value and fold-change criteria: blue dots: P value <0.05 with no cutoff on Absolute Log₂ Fold Change; red dots: P value <0.05 and Absolute Log₂ Fold Change value >1. (E) Metascape pathway analysis of genes significantly upregulated or downregulated in Atg5^ΔCd207 LCs. (F and G) Differentially expressed genes related to (F) apoptosis and (G) ferroptosis KEGG pathways. Blue boxes: downregulated in Atg5^ΔCd207 LCs, red boxes: upregulated in Atg5^ΔCd207 LCs; dashed boxes: FDR > 0.1.