ATG5-deficient Langerhans cells remain immature and undergo apoptosis. (A and B) CD86 (A) and MHCII (B) MFI for epidermal LCs of 3-wk-old control (Atg5^WT and Atg5^WT/Δ) and Atg5^ΔCd207 mice. Data are pooled from five independent experiments, with each point representing one individual mouse. Statistical analysis: Kruskal–Wallis one-way ANOVA followed by Dunn’s multiple comparison test (ns, P > 0.05). (C and D) Percentages (C) and absolute numbers (D) of LCs, cDC1, and CD207⁻ DCs in freshly digested skin draining lymph nodes of 3-wk-old control (Atg5^WT and Atg5^WT/Δ) and Atg5^ΔCd207 mice. Data are pooled from at least three independent experiments, with each point representing one individual mouse. Statistical analysis: Mann–Whitney U test (*, P < 0.05; ns, P > 0.05). (E) Percentage of cells with activated caspase-3 in LCs of freshly digested back skin epidermis (left panel) and LCs, cDC1, and CD207- dermal DCs of skin-draining lymph nodes (right panel) for 3-wk-old control (Atg5^WT and Atg5^WT/Δ) and Atg5^ΔCd207 mice. Data are pooled from at least three independent experiments, with each point representing one individual mouse. Statistical analysis: Kruskal–Wallis one-way ANOVA followed by Dunn’s multiple comparison test (*, P < 0.05; **, P < 0.01; ns, P > 0.05).