ROS disrupts exocyst-mediated endosomal recycling of Delta ligands. (A–C) Whole ovary images of control, PHB1-RNAi, and PHB1-RNAi +RAB11(o/e) egg chambers (two copies of GAL4). (D–F) Immunofluorescence images of control, PHB1-RNAi, and PHB1-RNAi +RAB11(o/e) egg chambers stained with Delta antibodies. (G) Ovary size measurements of control, PHB1-RNAi, and PHB1-RNAi +RAB11(o/e) egg chambers (one copy of GAL4) (n = 20 ovaries). (H) cytosolic delta level measurements of control, PHB1-RNAi, and PHB1-RNAi +RAB11(o/e) egg chambers (n = 25 egg chambers). (I) A graph depicting the percentage of mid-oogenesis egg chambers that display Delta membrane localization or cytosolic Delta aggregates (N = 25 egg chamber). (J) Immunofluorescence images of control and RAB11-DN expressing egg chambers stained with Delta antibodies (red) Arrows indicate Delta puncta. Student’s t test was used for all pairwise comparisons, and one-way ANOVA was used for all experiments containing >2 sample groups. Error bars represent the standard deviation.