Mitochondrial dysfunction imairs the trafficking to specific receptors. (A) Images of egg chambers from control, PHB1-RNAI #2, and PHB1^TS1 temperature-sensitive mutants stained for Delta ligand green. (B) Gurken antibody-stained egg chambers from control and PHB1-RNAi females. (C–D’’) Immunostaining of control and PHB1-RNAi egg chambers depicting delta (red) localization and mCD8-GFP (green) localization. (E–F′) Images of PTP69D staining in control and PHB1-RNAi ovarioles. (G–J) Notch-ECD (green) staining images of control and PHB1-RNAi egg chambers. (K) Fluorescence levels from antibody-stained images of control and PHB1-RNAi ovarioles (n = 25). Student’s t test was used for all pairwise comparisons, and one-way ANOVA was used for all experiments containing >2 sample groups. Error bars represent the standard deviation.