Mitochondrial dysfunction impairs Notch signaling between germ cells and follicle cells. (A) A diagram depicting the mitosis to endocycle transition, a very early step in follicle cell differentiation, and its regulation by Notch signaling. (B and C) Ovarioles from dsRED-RNAi control and PHB1-RNAi females stained with Delta ligand antibodies (red) and DAPI. (D) Immunofluorescence images of control and PHB1-RNAi egg chambers stained with Delta Antibodies (Red), NRE-GFP reporter(green), and DAPI. (E and F) immunofluorescence images of dsRED-RNAi control and PHB1-RNAi ovarioles stained with peb antibodies (green) and DAPI. (G) A graph showing NRE-GFP expression in control and PHB1-RNAi egg chambers (n = 20 follicles). (H) A graph showing the differences in cytosolic delta levels (n = 20 follicles). (I) Peb expression levels in control and Phb1-RNAi ovarioles (n = 20). Student’s t test was used for all pairwise comparisons, and one-way ANOVA was used for all experiments containing >2 sample groups. Error bars represent the standard deviation.