Germline mitochondrial dysfunction disrupts Drosophila ooegenesis. (A–C) Whole ovary images from control, mataX2(2 copies)–> PHB-RNAi, and mataX1PhB-RNAi females. (D–F) DAPI staining images of control, mataX2 PhB-RNAi, and mataX1PhB-RNAi ovarioles. (G) PH3+ positive cell counts from control and PHB1-RNAi#2 ovarioles (n = 20). (H and I) PH3 staining images of control and PHB1-RNAi #2 ovarioles. (J) RNAi-knockdown efficiencies measured by Q-PCR from control and RNAi transgenes used in this manuscript (n = 3). (K) GFP antibody staining images from the mata-GAL4-> UAS-GFP ovarioles confirming the germline-specific expression of the driver. (L) Gene ontology enrichment analysis for genes Downregulated in the PHB1-RNAi follicles. (M) Gene ontology enrichment analysis for genes upregulated in the PHB1-RNAi follicles. (N) A volcano plot of RNA-seq data comparing mRNA expression between control follicles and PHB1-RNAi expressing oocytes (FDR < 0.05). (O) RNA seq- data examining the expression of genes involved with follicle cell specification and patterning in PHB1-RNAi follicles. (P) RNA-seq data examining the expression of ecdysone pathway genes in PHB-RNAI follicles. (Genes marked with a red fold change display an FDR < 0.05). The control used in all RNAi experiments is mata ->dsRED-RNAi. Student’s t test was used for all pairwise comparisons, and one-way ANOVA was used for all experiments containing >2 sample groups. Error bars represent the standard deviation.