Figure 1.
Germ cell Mitochondrial dysfunction prevents follicle cell differentiation. (A) A diagram describing the use of Drosophila oogenesis to examine the non-cell autonomous effects of germline mitochondrial dysfunction on somatic follicle cell development. (B–E) DAPI stained images of stage 10 and stage 14 egg chambers from control and PHB1-RNAi ovaries. (F and G) Ovarioles stained with DAPI and phosphohistone H3 (red) from the matα–>DsRed-RNAi (control) and matα (one copy)–>PHB1-RNAi females. F′ and G′ are zoomed-in images of egg chambers from F and G. (H) Follicle cell number counts from stage 10 egg chambers represented as a box and whisker plot. (n = 25 follicles). (I) Size measurements of stage 14 egg chambers. The line in the plot represents the average value. n = 10 egg chambers. (J) The number of PH3+ follicle cells from control and matα–>PHB1-RNAi females (n = 30 ovarioles). (K) Follicle cell nucleus size measurements of control and PHB1-RNAi stage 10 egg chambers (N = 20). (L and M) Images of Control and PHB1-RNAi follicle cell clones: blue represents DAPI, and green represents GFP from the follicle cell clone. (N) A graph representing the frequency of one to two cell clones in control RNAi clones and PHB1-RNAi clones. (O) RNA-seq data examining the expression of known germ cell-expressed genes. The precise fold change is overlayed on the heatmap. (Genes marked with a red fold change display an FDR < 0.05). LpR1, LpR2, and yl are known to be expressed in germ cells during late oogenesis (Stage 9–12). P) RNA-seq data examining the expression of known follicle cell-expressed genes (genes marked in red display an FDR < 0.05). The control used in all RNAi experiments is mata ->dsRED-RNAi All RNA seq-data represents three biological replicates. Student’s t test was used for all pairwise comparisons, and one-way ANOVA was used for all experiments containing >2 sample groups. Error bars represent the standard deviation.

Germ cell Mitochondrial dysfunction prevents follicle cell differentiation. (A) A diagram describing the use of Drosophila oogenesis to examine the non-cell autonomous effects of germline mitochondrial dysfunction on somatic follicle cell development. (B–E) DAPI stained images of stage 10 and stage 14 egg chambers from control and PHB1-RNAi ovaries. (F and G) Ovarioles stained with DAPI and phosphohistone H3 (red) from the matα–>DsRed-RNAi (control) and matα (one copy)–>PHB1-RNAi females. F′ and G′ are zoomed-in images of egg chambers from F and G. (H) Follicle cell number counts from stage 10 egg chambers represented as a box and whisker plot. (n = 25 follicles). (I) Size measurements of stage 14 egg chambers. The line in the plot represents the average value. n = 10 egg chambers. (J) The number of PH3+ follicle cells from control and matα–>PHB1-RNAi females (n = 30 ovarioles). (K) Follicle cell nucleus size measurements of control and PHB1-RNAi stage 10 egg chambers (N = 20). (L and M) Images of Control and PHB1-RNAi follicle cell clones: blue represents DAPI, and green represents GFP from the follicle cell clone. (N) A graph representing the frequency of one to two cell clones in control RNAi clones and PHB1-RNAi clones. (O) RNA-seq data examining the expression of known germ cell-expressed genes. The precise fold change is overlayed on the heatmap. (Genes marked with a red fold change display an FDR < 0.05). LpR1, LpR2, and yl are known to be expressed in germ cells during late oogenesis (Stage 9–12). P) RNA-seq data examining the expression of known follicle cell-expressed genes (genes marked in red display an FDR < 0.05). The control used in all RNAi experiments is mata ->dsRED-RNAi All RNA seq-data represents three biological replicates. Student’s t test was used for all pairwise comparisons, and one-way ANOVA was used for all experiments containing >2 sample groups. Error bars represent the standard deviation.

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