PI3P and Fab1-complex components localize symmetrically on the vacuole periphery. (A) Representative still images and PCC values showing EGFP-2xFYVE colocalization with the vacuolar marker Vph1-3xmCherry in WT cells (MHY252). Unpaired two-tailed t test was performed. n.s: non-significant with P = 0.9036. Data shown are from two independent experiments. (B) Representative still images and daughter vacuole to mother vacuole (DV/MV) ratio of Atg18, Atg18-Sloop, Fab1, Vac14, and Vac7 (MUK089, MUK091, SGY008, SGY009, and MUK117) are shown. The red dashed line marks the DV/MV value of 1, which indicates symmetric distribution on both vacuoles without a preference for a specific vacuole. See Materials and methods for details. (C) Representative still images and PCC values showing SnxA-GFP colocalization with Vph1-3xmCherry on the mother and daughter vacuoles of wildtype (WT) cells and fab1-ha or atg18∆ mutants (MHY286, MHY288, and MHY289). Data shown are from two independent experiments. In the box-and-whisker graphs, boxes show the 25th to 75th percentiles, whiskers show the 10th and 90th percentiles, and lines inside the boxes are the mean. In B and C, ordinary one-way ANOVA was performed with Fisher’s LSD test. ***P = 0.0002, ****P < 0.0001, ns: non-significant with P ≥ 0.7441. Scale bars are 3 μm. BF: bright field.