Figure S2. Translation of mitochondrial... | Rockefeller University Press

Figure S2.

$Translation of mitochondrial proteins is not affected in the tif51A-1 strain at permissive temperature. (A) Scheme with the employed area divisions for calculations. (B) The average polysomes/2n-3n ratio (P/2n-3n) is represented for each strain at both temperatures. (C) The average polysomes/2n-8n ratio (P/2n-8n) is represented for each strain at both temperatures. (D–F) The RNA from individual fractions of the polysomes profiles was extracted and the mRNA levels of PET9, TOM20, TOM70 (D), ATP1, CYC1, COX5A (E), ACT1, RPP2B, and EFT2 (F) were analyzed by RT-qPCR in the corresponding sections at restrictive temperature. (G–I) The RNA from individual fractions of the polyribosome profiles was extracted and the mRNA levels of TOM20, TOM70 (G), CYC1, COX5A (H), and ACT1 (I) were analyzed by RT-qPCR in the corresponding sections at permissive temperature. Data information: In B–I, results are presented as mean ± SD from three independent experiments. The statistical significance was measured by using a two-tailed paired Student’s t test relative to wild-type strain. *P < 0.05, **P < 0.001, ***P < 0.001. n.s means no significant differences.$

Translation of mitochondrial proteins is not affected in the tif51A-1 strain at permissive temperature. (A) Scheme with the employed area divisions for calculations. (B) The average polysomes/2n-3n ratio (P/2n-3n) is represented for each strain at both temperatures. (C) The average polysomes/2n-8n ratio (P/2n-8n) is represented for each strain at both temperatures. (D–F) The RNA from individual fractions of the polysomes profiles was extracted and the mRNA levels of PET9, TOM20, TOM70 (D), ATP1, CYC1, COX5A (E), ACT1, RPP2B, and EFT2 (F) were analyzed by RT-qPCR in the corresponding sections at restrictive temperature. (G–I) The RNA from individual fractions of the polyribosome profiles was extracted and the mRNA levels of TOM20, TOM70 (G), CYC1, COX5A (H), and ACT1 (I) were analyzed by RT-qPCR in the corresponding sections at permissive temperature. Data information: In B–I, results are presented as mean ± SD from three independent experiments. The statistical significance was measured by using a two-tailed paired Student’s t test relative to wild-type strain. *P < 0.05, **P < 0.001, ***P < 0.001. n.s means no significant differences.