Figure S1.
Proteomic analysis upon eIF5A depletion. (A) MDS-plot showing all replicates for each strain and condition studied in the proteomic analysis. (B) Growth of the wild-type and tif51A-1 strains was tested in YPD media at the indicated temperatures. (C) Biological process overrepresented in proteins up- or downregulated significantly in wild-type cells at 41°C. GO Term analysis was done using the STRING tool, and a total of 272 proteins up- (left) and 154 proteins downregulated (right) significantly in WT 41°C compared with WT 25°C were analyzed. (D) Biological process GO terms overrepresented in relative up- (left) or downregulated (right) proteins in tif51Ats versus WT. tif51Ats stands for the average values obtained for the two eIF5A temperature-sensitive strains (tif51A-1 and tif51A-3). GO term analysis was done using the STRING tool, in which a total of 292 proteins down- and 135 proteins upregulated significantly in at least one tif51Ats with respect to wild-type were analyzed. The web-based tool REVIGO was used to summarize the GO terms. Bubble color indicates the P value of the GO term in the input data set; bubble size indicates the frequency of the GO term in the underlying GO database. (E) Wild-type, tif51A-1, and tif51A-3 strains were cultured in YPD at 25°C until early exponential phase and transferred to 25°C, 37°C, or 41°C for 4 h. eIF5A and Por1 protein levels were determined by western blotting (left) and quantified (right). G6pdh levels were used as loading control. A representative image is shown from three independent experiments. Data information: In E, results are presented as mean ± SD from three independent experiments. The statistical significance was measured by using a two-tailed Student’s t test. *P < 0.05, **P < 0.001, ***P < 0.001. The asterisks located above the columns of the graph represent a comparison relative to 25°C conditions within the same strain. The asterisks found at the top of the bars additionally indicate comparisons between the indicated strains at the same treatment condition. n.s means no significant differences. Source data are available for this figure: SourceData FS1.

Proteomic analysis upon eIF5A depletion. (A) MDS-plot showing all replicates for each strain and condition studied in the proteomic analysis. (B) Growth of the wild-type and tif51A-1 strains was tested in YPD media at the indicated temperatures. (C) Biological process overrepresented in proteins up- or downregulated significantly in wild-type cells at 41°C. GO Term analysis was done using the STRING tool, and a total of 272 proteins up- (left) and 154 proteins downregulated (right) significantly in WT 41°C compared with WT 25°C were analyzed. (D) Biological process GO terms overrepresented in relative up- (left) or downregulated (right) proteins in tif51Ats versus WT. tif51Ats stands for the average values obtained for the two eIF5A temperature-sensitive strains (tif51A-1 and tif51A-3). GO term analysis was done using the STRING tool, in which a total of 292 proteins down- and 135 proteins upregulated significantly in at least one tif51Ats with respect to wild-type were analyzed. The web-based tool REVIGO was used to summarize the GO terms. Bubble color indicates the P value of the GO term in the input data set; bubble size indicates the frequency of the GO term in the underlying GO database. (E) Wild-type, tif51A-1, and tif51A-3 strains were cultured in YPD at 25°C until early exponential phase and transferred to 25°C, 37°C, or 41°C for 4 h. eIF5A and Por1 protein levels were determined by western blotting (left) and quantified (right). G6pdh levels were used as loading control. A representative image is shown from three independent experiments. Data information: In E, results are presented as mean ± SD from three independent experiments. The statistical significance was measured by using a two-tailed Student’s t test. *P < 0.05, **P < 0.001, ***P < 0.001. The asterisks located above the columns of the graph represent a comparison relative to 25°C conditions within the same strain. The asterisks found at the top of the bars additionally indicate comparisons between the indicated strains at the same treatment condition. n.s means no significant differences. Source data are available for this figure: SourceData FS1.

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