CaTAr2 and ANO1–GFP in NCL cells. (A) Airy scan images of the Co-expression of CaTAr2 and ANO1–GFP in NCL cells indicate the partial overlap between the two signals. (B) The colocalization is not influenced by store depletion. (C) Relative intensities along a virtual line scan (white arrows in A) reveal the colocalization of ANO1 and CaTAr2 but also enrichment in ANO1 at the edge of the CaTAr2-rich ER patch.