CaTAr1 effects on tunneling and localization of ANO1–GFP and mCh-STIM1. (A) Dose-dependent inhibition of tunneling by CaTAr1. The amplitude of the tunneling signal is plotted as a function of the GFP signal normalized from minimum to maximum signal in each dish. The data is sorted in bins of 10%. (B) The relative localization of ANO1–GFP and mCh-STIM1 in NCL cells after store depletion is similar to the endogenous ANO1 channel and suggests the separation of ANO1 from the STIM1 cluster. (C) TIRF imaging of ANO1–GFP and mCh-STIM1 during store depletion with CPA confirmed the separation of the two proteins at the PM. Ctr and CPA images are from the same region of interest.