Localization of MAPPER–GFP, E-Syt2-mCh, and TMEM24-mCh. (A) Localization of MAPPER–GFP, E-Syt2-mCh, and TMEM24-mCh at the whole cell level. (B) Bar chart summarizing the PCC of the three tethers relative to STIM1 (n = 7–23; one-way ANOVA). (C) Confocal images and line scans illustrate the colocalization of E-Syt2-mCh and TMEM24-mCh with STIM1-CFP clusters. The intensity plots are obtained from the lines depicted by the white arrows.