Effects of ML-SA5 treatment on PA-induced mitochondrial ROS elevations and Parkin puncta formation. (A) Quantitation of ML-SA5 effects on PA-induced mitochondrial ROS elevation in TFEB knockout (KO) HeLa cells. (B) Effects of ML-SA5 on PA-induced Parkin puncta formation in Parkin-mScarlet stable HUVECs. (C) Quantitative analysis of data as shown in B. (D) Effects of TRPML1 overexpression on Parkin puncta formation in PA-treated Parkin-mScarlet stable HUVECs. (E) Quantitative analysis of data as shown in D. In panels A, C, and E, ≥50 randomly selected cells from three experimental repeats were analyzed.