Figure S3.
The PtdIns(4,5)2 labeling in PC12 cells.(A) PtdIns(4,5)P2 label density in PC12 cell samples. Freeze-fracture replicas of formaldehyde-fixed PC12 cells were treated with SDS alone or with SDS and proteinase K before labeling. (B) PtdIns(4,5)P2 labeling in formaldehyde-fixed PC12 cells. Freeze-fracture replicas were treated with SDS and proteinase K. Bar, 0.2 μm. (C) Point pattern analysis of the PtdIns(4,5)P2 label distribution by L-function (n = 10). The result of formaldehyde-fixed PC12 cells by using SDS/proteinase K-treated freeze-fracture replicas was added to Fig. 4 C. (D) PtdIns(4,5)P2 label density in non-MCC areas of formaldehyde-fixed budding yeast. Comparison between freeze-fracture replicas treated with SDS alone or with SDS and proteinase K. (E) PtdIns(4,5)P2 labeling in formaldehyde-fixed budding yeast. Freeze-fracture replicas were treated with SDS and proteinase K. MCC/eisosomes (arrowheads). Bar, 0.2 μm.

The PtdIns(4,5)2 labeling in PC12 cells. (A) PtdIns(4,5)P2 label density in PC12 cell samples. Freeze-fracture replicas of formaldehyde-fixed PC12 cells were treated with SDS alone or with SDS and proteinase K before labeling. (B) PtdIns(4,5)P2 labeling in formaldehyde-fixed PC12 cells. Freeze-fracture replicas were treated with SDS and proteinase K. Bar, 0.2 μm. (C) Point pattern analysis of the PtdIns(4,5)P2 label distribution by L-function (n = 10). The result of formaldehyde-fixed PC12 cells by using SDS/proteinase K-treated freeze-fracture replicas was added to Fig. 4 C. (D) PtdIns(4,5)P2 label density in non-MCC areas of formaldehyde-fixed budding yeast. Comparison between freeze-fracture replicas treated with SDS alone or with SDS and proteinase K. (E) PtdIns(4,5)P2 labeling in formaldehyde-fixed budding yeast. Freeze-fracture replicas were treated with SDS and proteinase K. MCC/eisosomes (arrowheads). Bar, 0.2 μm.

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