Figure 4.
PtdIns(4,5)P2in PC12 cells. (A) Fluorescence labeling of PtdIns(4,5)P2 in the basal plasma membrane of PC12 cells. (a) Unroofed cell membrane, (b) SDS-treated freeze-fracture replica of quick-frozen cell, (c) SDS-treated freeze-fracture replica of cells fixed before quick-freezing. Bar, 0.5 μm. (B) SDS-FRL of PtdIns(4,5)P2 in the basal plasma membrane of PC12 cells. Bar, 0.2 μm. (C) Point pattern analysis of the PtdIns(4,5)P2 label distribution in SDS-FRL by Ripley’s K-function (n = 10). The result is presented in the normalized form or L-function.

PtdIns(4,5)P 2 in PC12 cells. (A) Fluorescence labeling of PtdIns(4,5)P2 in the basal plasma membrane of PC12 cells. (a) Unroofed cell membrane, (b) SDS-treated freeze-fracture replica of quick-frozen cell, (c) SDS-treated freeze-fracture replica of cells fixed before quick-freezing. Bar, 0.5 μm. (B) SDS-FRL of PtdIns(4,5)P2 in the basal plasma membrane of PC12 cells. Bar, 0.2 μm. (C) Point pattern analysis of the PtdIns(4,5)P2 label distribution in SDS-FRL by Ripley’s K-function (n = 10). The result is presented in the normalized form or L-function.

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