Figure 3.
![PtdIns(4,5)P2in the stationary phase. (A) Total cellular content of PtdIns(4,5)P2 quantified by PRMC-MS (n = 3). (B) PtdIns(4,5)P2 shown by fluorescence biosensor GFP-PHPLCδ1. Bar, 10 μm. The relative FPM in the control and stationary phase. Mann–Whitney test (n = 42 [control], 41 [stationary phase]). ****P < 0.0001. (C) SDS-FRL of PtdIns(4,5)P2. Bar, 0.2 μm. (D) The label intensity by SDS-FRL in the control and stationary phase. Mann–Whitney test (stationary phase: n = 10 [total cell], 78 [curved MCC], 30 [shallow MCC], 15 [non-MCC]). ****P < 0.0001. The bar graph shows the proportion of labels in MCC/eisosomes and non-MCC/eisosome areas.](https://cdn.rupress.org/rup/content_public/journal/jcb/224/1/10.1083_jcb.202311067/1/jcb_202311067_fig3.png?Expires=1767804710&Signature=4TxfoIELASZVpdKssia-oqESMl0UFk2ba2jYSNAerKMRFw2oRPeeVDcRLPCUS7D9KtECAk0d6aCV7Pv7V2j7uAmZKsjSQaXYHi1AxRi6k77ifFkUl~ES-ReN1WBrmIViFTUpe1vr-LtCAYYPKIx1NTH2YTQpqVB64ssjLGhZTqI4LtrnX7n5QtBPbTrNkTPAP3SRfsLFBW9rINNMlbPRaTZHFVWR23P4lTqo5baGmxQutrhU5SQj13I4GMIaLUrYvpe7nGnZ20V-edEa1qixRhWc94MWmdnKDBXGqyjXyETOtngunLutofVsS37LRuu3IwdjePqhtGbEJE8ucjzeUg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PtdIns(4,5)P 2 in the stationary phase. (A) Total cellular content of PtdIns(4,5)P2 quantified by PRMC-MS (n = 3). (B) PtdIns(4,5)P2 shown by fluorescence biosensor GFP-PHPLCδ1. Bar, 10 μm. The relative FPM in the control and stationary phase. Mann–Whitney test (n = 42 [control], 41 [stationary phase]). ****P < 0.0001. (C) SDS-FRL of PtdIns(4,5)P2. Bar, 0.2 μm. (D) The label intensity by SDS-FRL in the control and stationary phase. Mann–Whitney test (stationary phase: n = 10 [total cell], 78 [curved MCC], 30 [shallow MCC], 15 [non-MCC]). ****P < 0.0001. The bar graph shows the proportion of labels in MCC/eisosomes and non-MCC/eisosome areas.