Figure 2.
![PtdIns(4,5)P2in yeast after acute ATP depletion. (A) Total cellular content of PtdIns(4,5)P2 quantified by PRMC-MS (n = 3). (B) PtdIns(4,5)P2 shown by fluorescence biosensor GFP-PHPLCδ1. Bar, 10 μm. The relative FPM in the control and after ATP depletion. Mann–Whitney test (n = 42 [control], 41 [ATP depletion]). ****P < 0.0001. (C) SDS-FRL of PtdIns(4,5)P2. Bar, 0.2 μm. (D) The label intensity by SDS-FRL in the control and after ATP depletion. Mann–Whitney test (ATP depletion: n = 10 [total cell], 58 [curved MCC], 16 [shallow MCC], and 15 [non-MCC]). ****P < 0.0001. The bar graph shows the proportion of labels in MCC/eisosomes and non-MCC/eisosome areas.](https://cdn.rupress.org/rup/content_public/journal/jcb/224/1/10.1083_jcb.202311067/1/jcb_202311067_fig2.png?Expires=1767804663&Signature=JgsbWkFbeP6lixwYZTt1xgrazLBQ6NhG8y6AV7uZVJZ1b0zq6S5IHKhLzGza5egqimT~z6BCTI4Mvk-XuoC7VXoSkf0QNnr8k346cyxAGWvAGjwTxQQRtPSAOZFyeSCb0zof7-ZEdbU2w-gjI9yOPxjpi3JwLF9rp8zOPFDWxQMNS73VXli-PaVEGrkV8xQ~a98LO~uW0uq99Tl7-NYX2Rh-zckmT335JhEvhuvMu~ddp~tkMVfHrE9pRHsnXQu5pnXrz5KybaYmZ3RtGnXdItOhSLsK4rDV8cqBeU1s7QDQQXL8gmGLsNDywjKGrbBGNHhmGc3kX8ElpTSjt9fBxQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
PtdIns(4,5)P 2 in yeast after acute ATP depletion. (A) Total cellular content of PtdIns(4,5)P2 quantified by PRMC-MS (n = 3). (B) PtdIns(4,5)P2 shown by fluorescence biosensor GFP-PHPLCδ1. Bar, 10 μm. The relative FPM in the control and after ATP depletion. Mann–Whitney test (n = 42 [control], 41 [ATP depletion]). ****P < 0.0001. (C) SDS-FRL of PtdIns(4,5)P2. Bar, 0.2 μm. (D) The label intensity by SDS-FRL in the control and after ATP depletion. Mann–Whitney test (ATP depletion: n = 10 [total cell], 58 [curved MCC], 16 [shallow MCC], and 15 [non-MCC]). ****P < 0.0001. The bar graph shows the proportion of labels in MCC/eisosomes and non-MCC/eisosome areas.