Figure S2.
![The PtdIns(4,5)P2 labeling in yeast and liposomes.(A) Distribution of Pil1-GFP observed by conventional fluorescence microscopy. Bar, 10 μm. (B) PtdIns(4,5)P2 labeling by SDS-FRL. MCC (arrows). Bars, 0.5 mm (left), 0.2 μm (right). (C) PtdIns(4,5)P2 labeled by two different concentrations of GST-PHPLCδ1. Bar, 0.5 μm. (D) Liposomes containing 5% PtdIns(4,5)P2 and 1% Liss Rhod phosphatidylethanolamine incubated with or without GFP-PHPLCδ1. Bar, 1 μm. (E) SDS-FRL of PtdIns(4,5)P2-containing liposomes with or without GFP-PHPLCδ1. Bar, 0.2 μm. The PtdIns(4,5)P2 label density in the liposome by SDS-FRL. Mann–Whitney test (n = 27 [without GFP-PHPLCδ1], 25 [with GFP-PHPLCδ1]).](https://cdn.rupress.org/rup/content_public/journal/jcb/224/1/10.1083_jcb.202311067/1/jcb_202311067_figs2.png?Expires=1767803090&Signature=GqIdSPzU8vM3diSxJxl7CqS~lrLeau2FJQVeikwNrXHSGJ4ccBwu4ik6ahjiRy-7ZccOwS-Zhc81fhCwe-yf6VrDdxhVyzN2Rlp1wtVLLKmRerTdFAq4vhgKXxthfaMefVg6PA1nUqlpX7ICqzON5R5vEhRz-VRoxmUDioTS6XwVTvBh7nrL9lS-Nz66NLK6YTqLwIdD4klzpX~7IoktUY0VKmu-IZNzZbmO~p8FtcDZXicU1rdk1I867aYDdFusRm9S6umT0yAYvpm1JLDy1wvO7DXfut4Xw3ttFlceRXJC2wFNMsKsbsAyjQg2Bhk9xtWySs3QfVSZF9fqfNGRkw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The PtdIns(4,5)P2 labeling in yeast and liposomes. (A) Distribution of Pil1-GFP observed by conventional fluorescence microscopy. Bar, 10 μm. (B) PtdIns(4,5)P2 labeling by SDS-FRL. MCC (arrows). Bars, 0.5 mm (left), 0.2 μm (right). (C) PtdIns(4,5)P2 labeled by two different concentrations of GST-PHPLCδ1. Bar, 0.5 μm. (D) Liposomes containing 5% PtdIns(4,5)P2 and 1% Liss Rhod phosphatidylethanolamine incubated with or without GFP-PHPLCδ1. Bar, 1 μm. (E) SDS-FRL of PtdIns(4,5)P2-containing liposomes with or without GFP-PHPLCδ1. Bar, 0.2 μm. The PtdIns(4,5)P2 label density in the liposome by SDS-FRL. Mann–Whitney test (n = 27 [without GFP-PHPLCδ1], 25 [with GFP-PHPLCδ1]).