PtdIns(4,5)P ₂ in log phase yeast. (A) Freeze-fracture images of curved MCC (green) and shallow MCC (red). Bar, 0.1 μm. (B) Distribution of Sur7-GFP and Pma1-GFP shown by SDS-FRL. Bar, 0.2 μm. (C) PtdIns(4,5)P₂ distribution indicated by fluorescence biosensor GFP-PH^PLCδ1. Bar, 10 μm. (D) PtdIns(4,5)P₂ distribution shown by SDS-FRL. Curved MCC (green) and shallow MCC (red) are colored. Bar, 0.2 μm. (E) The PtdIns(4,5)P₂ label density by SDS-FRL. The results in the total cell membrane (n = 10), curved MCC (n = 66), shallow MCC (n = 9), and non-MCC areas (n = 15) are shown. (F) The PtdIns(4,5)P₂ label density was measured at intervals of 25 nm from the center line of MCC/eisosomes. Left: schematic of the method. Right: results for shallow and curved MCC/eisosomes. The number of MCC/eisosomes measured: 69 (shallow), 71 (curved). (G) Yeast replica labeled with a mutant probe, GST- PH^{PLCδ1(K30N,K32N)}. Bar, 0.2 μm. (H) The density of PtdIns(4,5)P₂ labels in different cells. The colored circles indicate the average density of the labels in each cell. Number of cells counted: 10.