Figure 7.
Acute deletion of H2-Aa or Ciita or antibody-mediated blockade of MHC-II inhibited B16F10 melanoma growth. (A–C) Tumor growth curves of B16F10 melanoma after s.c. inoculation on day 0 into the flank of mice. (A) Effect on tumor growth of tamoxifen-induced deletion of H2-Aa in the host. 100 mg tamoxifen/kg body weight was injected i.p. daily on days 5–7 (n = 4 per group). (B) Effect on tumor growth of monoclonal antibody (HB12-18) against MHC-II. Different concentrations of HB12-18 were injected i.p. into WT mice on the indicated days (n = 5 per group). (C) Effect on tumor growth of combined anti-PD1 (10 μg/g body weight) and HB12-18 (25 μg/g body weight) treatment. Antibodies were injected i.p. on days 3, 5, 7, and 10 into WT mice (n = 5 per group). (D) Survival curves of mice after i.v. inoculation with B16F10 melanoma on day 0. WT mice were intraperitoneally (i.p.) injected with HB12-18 (50 μg/g body weight) and anti-PD1 twice per week till the end of the experiment (death or euthanasia) (n = 10 per group). (E) Frequency of tumor infiltrating lymphocytes and CD8 T cells in B16F10 tumors collected on day 11 after B16F10 inoculation. Control IgG or HB12-18 monoclonal antibodies were injected i.p. into WT mice on days 3, 5, 7, and 10 (n = 5 per group). (F) WT mice were s.c inoculated with B16F10 on day 0 and i.p injected with control IgG or HB12-18 on days 3, 5, 7, and 10. On day 11, splenic CD8 T cell and CD4 T cell activation were analyzed by flow cytometry (n = 5 per group). (G) Tumor growth curves of B16F10 melanoma after s.c. inoculation on day 0 into the flank of mice. 100 mg tamoxifen/kg body weight was injected i.p. daily on days 5–7 (n = 7–15 per group). Data are representative of two independent experiments (A–G). WT C57BL/6J mice from JAX were used as controls (A and G). Error bars indicated SEM (A–C and G) or SD (E and F). P values were determined by two-way ANOVA with post-hoc Tukey test (A–C and G), Student’s t test (E and F), or log-rank test (D). Data points represent individual mice (E and F). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Acute deletion of H2-Aa or Ciita or antibody-mediated blockade of MHC-II inhibited B16F10 melanoma growth. (A–C) Tumor growth curves of B16F10 melanoma after s.c. inoculation on day 0 into the flank of mice. (A) Effect on tumor growth of tamoxifen-induced deletion of H2-Aa in the host. 100 mg tamoxifen/kg body weight was injected i.p. daily on days 5–7 (n = 4 per group). (B) Effect on tumor growth of monoclonal antibody (HB12-18) against MHC-II. Different concentrations of HB12-18 were injected i.p. into WT mice on the indicated days (n = 5 per group). (C) Effect on tumor growth of combined anti-PD1 (10 μg/g body weight) and HB12-18 (25 μg/g body weight) treatment. Antibodies were injected i.p. on days 3, 5, 7, and 10 into WT mice (n = 5 per group). (D) Survival curves of mice after i.v. inoculation with B16F10 melanoma on day 0. WT mice were intraperitoneally (i.p.) injected with HB12-18 (50 μg/g body weight) and anti-PD1 twice per week till the end of the experiment (death or euthanasia) (n = 10 per group). (E) Frequency of tumor infiltrating lymphocytes and CD8 T cells in B16F10 tumors collected on day 11 after B16F10 inoculation. Control IgG or HB12-18 monoclonal antibodies were injected i.p. into WT mice on days 3, 5, 7, and 10 (n = 5 per group). (F) WT mice were s.c inoculated with B16F10 on day 0 and i.p injected with control IgG or HB12-18 on days 3, 5, 7, and 10. On day 11, splenic CD8 T cell and CD4 T cell activation were analyzed by flow cytometry (n = 5 per group). (G) Tumor growth curves of B16F10 melanoma after s.c. inoculation on day 0 into the flank of mice. 100 mg tamoxifen/kg body weight was injected i.p. daily on days 5–7 (n = 7–15 per group). Data are representative of two independent experiments (A–G). WT C57BL/6J mice from JAX were used as controls (A and G). Error bars indicated SEM (A–C and G) or SD (E and F). P values were determined by two-way ANOVA with post-hoc Tukey test (A–C and G), Student’s t test (E and F), or log-rank test (D). Data points represent individual mice (E and F). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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