Lymphocyte frequencies in mixed bone marrow chimeric mice. (A–E) A 1:1 mixture of H2-Aacit/cit (cit, CD45.2) or WT (CD45.2) bone marrow cells and congenic WT (CD45.1) bone marrow cells were transferred to lethally irradiated WT (CD45.1) recipients (n = 5 or 6 recipients per group). (A) Representative flow cytometry plots of the indicated cDC populations in mesenteric lymph nodes (MLN) of bone marrow chimeric mice. Reduced cDC1 and increased cDC2 frequencies were observed among cDC derived from CD45.2 H2-Aacit/cit BM (cit, highlighted in red). (B) Expression of MHC-I (upper panel) and CD80 (lower panel) on MLN cDC2 from bone marrow chimeric mice. MFI, mean fluorescence intensity. (C and D) Frequencies of B cells, CD4 T cells, CD8 T cells (C), and Treg (D) in the spleens of bone marrow chimeric mice. (E) Frequency of Treg in tumor infiltrating lymphocytes from B16F10 melanoma collected on day 21 after B16F10 inoculation in the flank of bone marrow chimeric mice. (F) Tumor growth curves of B16F10 melanoma after s.c. inoculation on day 0 into the flank of WT mice. Naïve cDC2 cells were intratumorally injected on day 3 (n = 6–8 per group). Data points represent individual mice (B–E). Data are representative of two independent experiments (A–F). Error bars indicate SD (B–E) or SEM (F). P values were determined by Student’s t test (B–E) or two-way ANOVA with post-hoc Tukey test (F). *P < 0.05; **P < 0.01; ****P < 0.0001; ns, not significant.