Figure S3.
TRPV1-T468tag-GFP and TRPV1-K464tag-GFP negative control. Expression of functional TRPV1 channels is qualitatively absent when assessing Ca2+ imaging responses to 500 nM capsaicin without 30 μM TCO (ncAA) in media. (A and B) Neither TRPV1-T468tag-GFP (A) nor TRPV1-K464tag-GFP (B) generate Ca2+ responses in comparison to their transient transfection expression controls when supplemented with the pNEU plasmid but grown without TCO. All fluorescence experiment image sets are normalized to the brightness of their respective ionomycin image.

TRPV1-T468tag-GFP and TRPV1-K464tag-GFP negative control. Expression of functional TRPV1 channels is qualitatively absent when assessing Ca2+ imaging responses to 500 nM capsaicin without 30 μM TCO (ncAA) in media. (A and B) Neither TRPV1-T468tag-GFP (A) nor TRPV1-K464tag-GFP (B) generate Ca2+ responses in comparison to their transient transfection expression controls when supplemented with the pNEU plasmid but grown without TCO. All fluorescence experiment image sets are normalized to the brightness of their respective ionomycin image.

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