Protein expression of TRPV1-tag constructs. (A) Testing of construct TRPV1-Q169tag in the presence and absence of RNA synthetase/tRNA plasmid (pNEU) + ncAA (TCO). Lanes 5, 6, and 7 contain expression products of TRPV1-K240tag, T468tag, and V469tag without pNEU. All lanes are probed with GFP antibody. (B) Testing of TRPV1-V469 in the presence and absence of pNEU and two different ncAAs (TCO or LysZ). Lanes are probed with the GFP antibody. Source data are available for this figure: SourceData FS1.