The effect of OA and LA on the relative mRNA expression levels of irs1. (A) Relative mRNA levels of irs1 were tested by quantitative PCR in the muscle of fish fed CON, OO, or LO diet (n = 4 fish per group). (B) Relative mRNA levels of irs1 were analyzed by quantitative PCR in C2C12 myotubes under the indicated concentrations of OA or LA treatment for 12 h (n = 3 independent wells per treatment). The results are presented as the mean ± SEM and were analyzed using independent t tests (*P < 0.05).