OA and LA have no effect on mTORC1 activity. (A and B) mTORC1 pathway activity was measured by immunoblotting for the phosphorylation of S6K and S6 in skeletal muscle of fish fed CON, OO, or LO diet for 10 wk (n = 6 fish per group). (C and D) mTORC1 pathway activity was analyzed by immunoblotting in fish myocytes treated with the indicated concentrations of OA or LA for 12 h (n = 3 independent wells per treatment). The results are presented as the mean ± SEM and were analyzed using independent t tests (*P < 0.05). Source data are available for this figure: SourceData FS2.