CTLA4 expression in cancer cell lines. (A) mRNA levels derived from the Cancer Cell Line Encyclopedia (CCLE) database (Barretina et al., 2012). Each dot represents an individual tumor cell line within the indicated categories. (B) Representative western blot of NCI-H520 and A2058 cells transfected with siRNA against non-targeting (NT1) or CTLA4 for 72 h prior to lysis. (C) Representative western blot of HeLa S3 Flp-In CTLA4-HA cells treated with Dynasore (80 µM) for 30 min in serum-free media before the addition of Cycloheximide (CHX, 100 µg/ml) for 2 h. (D) Quantification of CTLA4-HA levels for data represented in C. Individual data points from three independent, color-coded experiments are shown. Error bars indicate SD. One-way ANOVA and Dunnett’s multiple comparison test, **P < 0.01, ****P < 0.0001. Source data are available for this figure: SourceData FS1.
Figure S1.

CTLA4 expression in cancer cell lines. (A) mRNA levels derived from the Cancer Cell Line Encyclopedia (CCLE) database (Barretina et al., 2012). Each dot represents an individual tumor cell line within the indicated categories. (B) Representative western blot of NCI-H520 and A2058 cells transfected with siRNA against non-targeting (NT1) or CTLA4 for 72 h prior to lysis. (C) Representative western blot of HeLa S3 Flp-In CTLA4-HA cells treated with Dynasore (80 µM) for 30 min in serum-free media before the addition of Cycloheximide (CHX, 100 µg/ml) for 2 h. (D) Quantification of CTLA4-HA levels for data represented in C. Individual data points from three independent, color-coded experiments are shown. Error bars indicate SD. One-way ANOVA and Dunnett’s multiple comparison test, **P < 0.01, ****P < 0.0001. Source data are available for this figure: SourceData FS1.

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