NHR-49, HLH-30, and DAF-16 are not required for the transcriptional downregulation of acdh-1 and hach-1 in epidermis. (A and B) Quantification of the acdh-1 transcriptional activity in different strains during fasting. For % of nuclei with RNA spots, n represents the number of animals. For the intensity of a single RNA spot, n represents the number of alleles. n represents the number of nuclei when analyzing the number of transcriptional active alleles per nucleus and the total intensity of all alleles per nucleus. The exact numbers analyzed were indicated above each bar. Values are displayed as mean ± SEM. (C–E) Bar graph showing the quantifications of the hach-1 transcriptional activity in different strains during fasting. The meaning of n value is the same as shown in A and B. The exact numbers analyzed were indicated above each bar. All values are displayed as mean ± SEM. ns: not significant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (comparison between multiple groups with the same genotype: one-way ANOVA with the Tukey correction; comparison between two groups with different genotypes: two-tailed unpaired Student’s t test).