Interactions between the Ft ICD and core complex proteins in S2 cells. (A) Recruitment of Dachs:V5, App:GFP, and Dlish:HA by Ed:Ft:Flag in S2 cells. All three proteins accumulate with the Ft ICD at cell contacts (yellow arrows). (B) Depletion of app and dlish by RNAi does not affect the ability of the Ft ICD to recruit Dachs:GFP (yellow arrows). (C) Co-IP experiments similarly show that the Ft ICD forms a complex with Dachs in S2 cells even when App and Dlish are depleted. (D) Structure cartoons of Ft ICD deletions designed to identify regions responsible for core complex recruitment in S2 cells. Functional domains and App and Dlish association sites from previous studies (Matakatsu and Blair, 2012; Misra and Irvine, 2016; Zhang et al., 2016) are also shown. TM, transmembrane domain; PH, planar cell polarity (PCP)-Hippo; ND, not determined. (E–G) S2 cell assays for recruitment of core complex proteins by different Ft ICD deletions. (E) Ed:FtΔPH-C:Flag fails to recruit core complex proteins to cell contacts. Ed:FtΔ6-C:Flag (F) as well as Ed:FtΔΝ-6:Flag (G) recruit Dachs:GFP and Dlish:HA (yellow arrows). (H) Co-IP experiments confirm the results in E–G. Scale bars, (A, B, and E–G) 5 µm. Source data are available for this figure: SourceData F7.