Effects of the Ft ICD on Dachs and App. (A–C) Representative wing discs of the following genotypes stained with phalloidin: wild-type (A), ft^G-rv/ft^fd (B), and ft^ΔICD (C). Note that ft^ΔICD has a more severe overgrowth phenotype than ft null mutants. Panels B and C were stitched together because of their large size. (D) Localization of Dachs and Ds in ft^ΔICD mutant cells. Dachs:GFP expression is elevated and uniformly distributed around the junctional cortex, while Ds is only slightly elevated and localizes more normally at the junctions. Note that Dachs does not display the non-junctional puncta that are seen in ft null mutant cells (Fig. 1 J″). (E) Localization and abundance of App:YFP is not altered in ft^ΔICD mutant cells. (F) Dachs and App:YFP levels are elevated but localization to junctional puncta does not change in ft^sum mutant cells. (G) App:YFP abundance also is elevated at junctional puncta in dco³ mutant cells. (H–M) Surface labeling of cell clones expressing HA:Ds (H and I), App:V5 (I, K, and M), or V5:Ft (J and L) together with the indicated tagged proteins. All clones show border non-expressing sister clones. (H) Dachs:GFP puncta colocalize with HA:Ds at clone boundaries. (I) Similarly, HA:Ds in puncta colocalizes with App:V5. (J) In contrast, V5:Ft puncta do not colocalize with Dachs:GFP at clone boundaries (yellow ellipses). (K) Similarly, Ft:GFP puncta do not co-stain for App:V5 at clone boundaries (yellow ellipses). Additionally, Ft:GFP fails to colocalize with App:V5 puncta at clone boundaries on the opposite side of cells (red ellipses). (L) In contrast to wild-type, in a dco³ mutant background, V5:Ft and Dachs:GFP colocalize in puncta at clone boundaries (yellow circles). (M) Similarly, in a dco³ mutant background, App:V5 and Ft:GFP extensively colocalize in puncta at clone boundaries (yellow ellipses). Scale bars, (A–C) 100 µm, (D–M) 5 µm.