Mapping functional domains in the Ds ICD. (A–D) Anti-Ds staining in ds^ΔA:Flag, ds^ΔB:Flag, ds^ΔC:Flag, and ds^ΔD:Flag clones. Mutant clones are labeled by the absence of GFP. The level and distribution of Ds did not change in ds deletion mutants. (E–H) Quantification of fluorescence intensity along cell junctions showing colocalization of Dachs with Ds^:Flag and its deletions. Dachs^:Flag (E) and Ds^ΔA:Flag (F) show stronger colocalization with Dachs. Ds^ΔB:Flag (G) and Ds^ΔD:Flag (H) show weaker colocalization with Dachs. (I–K) Wings of the indicated genotypes showing transheterozygous complementation. ds^ΔA:Flag/ds^ΔB:Flag and ds^ΔA:Flag/ds^ΔD:Flag wings are almost normal size (I and J). ds^ΔB:Flag/ds^ΔD:Flag wings display slight undergrowth (K). (L–N) Leg phenotype in transheterozygous combinations. Boundaries of tarsal segments are marked with black lines. ds^ΔA:Flag/ds^ΔB:Flag (L) and ds^ΔA:Flag/ds^ΔD:Flag (M) legs display normal segmentation. ds^ΔB:Flag/ds^ΔD:Flag legs (N) are fused at T2 and T3 (yellow arrow). Scale bars, 5 µm (A–D), 1 µm (E–H), 100 µm (I–K and L–N).