The Ds ICD influences Dachs localization. (A) Ds:GFP and Dachs colocalize at junctional puncta (green arrows) in wing discs. (B) Fluorescence intensity of Dachs and Ds:GFP along the yellow line in A showing strong colocalization. (C) In ds^ΔICD clones, Dachs:GFP loses its punctate appearance and becomes more uniformly distributed along the junctional cortex (the absence of nuclear RFP serves as clonal marker). (D) Localization of Dachs and Ds^ΔICD:GFP in a ds^ΔICD:GFP homozygote. Dachs fails to colocalize with Ds^ΔICD:GFP (green arrows indicate Ds^ΔICD:GFP puncta, red arrows indicate Dachs puncta). (E) Fluorescence intensity of Dachs and Ds^ΔICD:GFP along the yellow line in D showing weak colocalization in comparison to B (Pearson’s correlation coefficient r = 0.135 versus r = 0.795). (F–F″) Dachs:GFP accumulates strongly and almost exclusively at the junctional cortex in ds^ΔICDft mutant clones. Note the absence of non-junctional puncta of Dachs:GFP here in comparison to ft mutant cells in Fig. 1, J″. Scale bars, (A, C″, C‴, D, and F″) 1 µm, (C and F) 5 µm.