Figure S3.
Nuclear behaviors in MT-disrupted embryos. (A) Still images showing centrosomes (green) and nuclei (magenta) (top) highlighting the nuclear notches (bottom) where centrosomes reside. (B) Comparison of relative myosin intensities at horizontal (75–90°) and vertical (0–15°) interfaces for control (vehicle-injected) and MT- (colchicine-injected) embryos; n = 2,289 interfaces for control and n = 2,342 interfaces for MT- from k = 3 embryos. Insets show rose plots indicating the directionality of the contracting interface, n = 2,430 interfaces for control and n = 1,043 interfaces for MT- from k = 3 embryos for each background. (C) Probability of absolute position of nuclear midplane along the apical–basal axis at 0 and 20 min of GBE for control and MT- embryos; n = 125 nuclei for 0 min and n = 132 nuclei for 20 min in control and n = 88 for 0 min and n = 98 for 20 min in MT- from k = 1 embryo for each background. (D) The volume of nucleus in MT- embryos compared at the early (0 min) and mid- (+20 min) GBE; n = 372 nuclei from k = 3 embryos. (E) Area of nuclei at their midplanes in MT- embryos compared at early (0 min) and mid- (+20 min) GBE. (F) Comparison of cell area of MT- embryos at a given z-plane at early (0 min) and mid- (+20 min) GBE. For D–F, n = 290 nuclei and cells from k = 3 embryos. (G) Maximum velocity at which nucleus move in apical–basal axis compared early (0 min) and mid- (+20 min) GBE in MT- embryos (left); n = 246 nuclei for early and n = 220 nuclei for mid-GBE from k = 3 embryos and on the right, comparing average speed of nucleus in control and MT- embryos; n = 554 nuclei for control and n = 326 nuclei for MT- from k = 3 embryos for each background. (H) Comparing gross extension of tissue measured by pole cell migration along the AP axis in control and MT- embryos; n = 6 (control) and n = 8 (MT-) embryos. (I) Probability of absolute position of nuclear midplane along the apical–basal axis at 0 and 20 min of GBE for kuk control (vehicle-injected kuk) and kuk MT- (colchicine-injected kuk) embryos; for kuk control, n = 215 nuclei for 0 min and n = 127 nuclei for 20 min, for kuk MT-, n = 88 for 0 min and n = 76 for 20 min from k = 1 embryo for each background. (J) Maximum packing ratio achieved by clusters of nuclei as measured at pre- (−5 min) and mid- (+20 min) GBE for kuk MT- embryos; n = 706 clusters of cells (ranging from 198 to 276 per embryo) from k = 3 embryos. The measured n values are regardless of timepoints where not indicated. For D–F, statistical significance was calculated using Student’s t test. For B and G–I, statistical significance was calculated using the Mann–Whitney U-test. ns = not significant, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Nuclear behaviors in MT-disrupted embryos. (A) Still images showing centrosomes (green) and nuclei (magenta) (top) highlighting the nuclear notches (bottom) where centrosomes reside. (B) Comparison of relative myosin intensities at horizontal (75–90°) and vertical (0–15°) interfaces for control (vehicle-injected) and MT- (colchicine-injected) embryos; n = 2,289 interfaces for control and n = 2,342 interfaces for MT- from k = 3 embryos. Insets show rose plots indicating the directionality of the contracting interface, n = 2,430 interfaces for control and n = 1,043 interfaces for MT- from k = 3 embryos for each background. (C) Probability of absolute position of nuclear midplane along the apical–basal axis at 0 and 20 min of GBE for control and MT- embryos; n = 125 nuclei for 0 min and n = 132 nuclei for 20 min in control and n = 88 for 0 min and n = 98 for 20 min in MT- from k = 1 embryo for each background. (D) The volume of nucleus in MT- embryos compared at the early (0 min) and mid- (+20 min) GBE; n = 372 nuclei from k = 3 embryos. (E) Area of nuclei at their midplanes in MT- embryos compared at early (0 min) and mid- (+20 min) GBE. (F) Comparison of cell area of MT- embryos at a given z-plane at early (0 min) and mid- (+20 min) GBE. For D–F, n = 290 nuclei and cells from k = 3 embryos. (G) Maximum velocity at which nucleus move in apical–basal axis compared early (0 min) and mid- (+20 min) GBE in MT- embryos (left); n = 246 nuclei for early and n = 220 nuclei for mid-GBE from k = 3 embryos and on the right, comparing average speed of nucleus in control and MT- embryos; n = 554 nuclei for control and n = 326 nuclei for MT- from k = 3 embryos for each background. (H) Comparing gross extension of tissue measured by pole cell migration along the AP axis in control and MT- embryos; n = 6 (control) and n = 8 (MT-) embryos. (I) Probability of absolute position of nuclear midplane along the apical–basal axis at 0 and 20 min of GBE for kuk control (vehicle-injected kuk) and kuk MT- (colchicine-injected kuk) embryos; for kuk control, n = 215 nuclei for 0 min and n = 127 nuclei for 20 min, for kuk MT-, n = 88 for 0 min and n = 76 for 20 min from k = 1 embryo for each background. (J) Maximum packing ratio achieved by clusters of nuclei as measured at pre- (−5 min) and mid- (+20 min) GBE for kuk MT- embryos; n = 706 clusters of cells (ranging from 198 to 276 per embryo) from k = 3 embryos. The measured n values are regardless of timepoints where not indicated. For D–F, statistical significance was calculated using Student’s t test. For B and G–I, statistical significance was calculated using the Mann–Whitney U-test. ns = not significant, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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